The head of the lab, Dr. D, jokingly asked me this when checking in on my progress and the short answer is no.
The long answer still ends in a resounding no but is a bit more intricate. After identifying the candidate genes by cross referencing the two lists of RNA binding factors and Nonsense Mediated Decay proteins I had 6 genes. My next step was to research those genes to see if they were at all involved with Cox - 2 a gene that's prevalence is heavily reduced whenever the integrin alpha 3 is knocked down. For most of them the answer was no but for a couple the answer was yes. I also researched their general function and was surprised to find out that some genes had functions as far reaching as embryonic development and when overexpressed leukimia. After doing that research the next steps would be to order primers for those 6 genes and run PCRs against alpha 3 to see if they really are down regulated as I predicted from cross referencing the two lists.
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Michaela BentonI'm lucky enough to go to this amazing school that has this amazing program that lets me learn amazing things. Archives
December 2017
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