Today I sat down with Rakshitha for the first time and she basically gave me a mini cancer cell biology lesson with the pertinent information about what I would be doing.
The first thing she went over was how to use NCBI's Blast function on their website. I'll need this tool later on. Blast compares DNA or RNA against different databases to see how similar they are while allowing you to specify what organism you want to find that specific DNA strand in. You can also use NCBI for proteins which is helpful because I'll be looking at RNA splicing factors which are a type of protein. The example she took me through was with something called ptgas 2. I needed to choose Fasta which is a file format that gives you DNA. You put the DNA sequence into BLAST. In blood choose non-redundant proteins and then reference proteins. Query is what you asked for while Subject is basically a hit or result. The E-value is the expected value. The lower the better because the higher it is the more chance that the match is just by chance. For example 2 x 10^-6 is bad.
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Michaela BentonI'm lucky enough to go to this amazing school that has this amazing program that lets me learn amazing things. Archives
December 2017
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