Throughout this month I got to observe Dr. R help a fellow lab researcher who was struggling to get results from a PCR due to trouble working with RNA. RNA is very unstable and easily broken down by the tiniest bit of contamination. I learned good lab behaviours which included spraying absolutely everything that you come into contact with RNAse spray to avoid contamination. You had to spray: the workbench, the pipette, even your gloves to avoid any possible contamination and still you might not get the results you're looking for due to contamination on quite literally the microscopic level. I first observed the protocol going from single stranded cDNA to RNA to primers to results until finally I thought I was ready to run my own PCR.
0 Comments
Leave a Reply. |
Michaela BentonI'm lucky enough to go to this amazing school that has this amazing program that lets me learn amazing things. Archives
December 2017
Categories
|